PKA C-alpha specific Polyclonal antibody

PKA C-alpha specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 55388-1-AP

Synonyms

PKA C alpha, PKACA, PRKACA

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Validation Data Gallery

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  WB analysis of human testis using 55388-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55388-1-AP (PKA C-alpha speicifc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IHC staining of human colon cancer using 55388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55388-1-AP (PKA C-alpha speicifc antibody at dilution of 1:50.

• 

  IP experiment of mouse brain using 55388-1-AP

  IP Result of anti-PKA C-alpha speicifc (IP:55388-1-AP, 4ug; Detection:55388-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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Tested Applications

• Positive WB detected in: human testis tissue, human liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below

Product Information

55388-1-AP targets PKA C-alpha specific in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase, cAMP-dependent, catalytic, alpha
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 38-43 kDa
• GenBank accession number: NM_002730
• Gene symbol: PRKACA
• Gene ID (NCBI): 5566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus. This antibody is specific to PRKACA.

Publications

Species	Application	Title
mouse	WB	Mol Med Rep; PMID: 30365072; Authors - Zhenyu Qiu
mouse	WB	Neural Plast; Neural Plasticity Associated with Hippocampal PKA-CREB and NMDA Signaling Is Involved in the Antidepressant Effect of Repeated Low Dose of Yueju Pill on Chronic Mouse Model of Learned Helplessness.; Authors - Zhilu Zou
		CNS Neurosci Ther; Increased Expression of Transient Receptor Potential Vanilloid 4 in Cortical Lesions of Patients with Focal Cortical Dysplasia.; Authors - Xin Chen
human	WB	Proteomics; Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein Upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.; Authors - Hwee Tong Tan
mouse	WB	Phytother Res; Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.; Authors - Lei Wu
mouse	WB	Front Pharmacol; An Integrative Pharmacology-Based Strategy to Uncover the Mechanism of Xiong-Pi-Fang in Treating Coronary Heart Disease with Depression.; Authors - Lihong Zhang