PKA C-alpha specific Polyclonal antibody

PKA C-alpha specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55388-1-AP

Synonyms

PKA C alpha, PKACA, PRKACA



Tested Applications

Positive WB detected inhuman testis tissue, human liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

55388-1-AP targets PKA C-alpha specific in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Calculated molecular weight 41 kDa
Observed molecular weight 38-43 kDa
GenBank accession numberNM_002730
Gene symbol PRKACA
Gene ID (NCBI) 5566
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus. This antibody is specific to PRKACA.

Protocols

Product Specific Protocols
WB protocol for PKA C-alpha specific antibody 55388-1-APDownload protocol
IHC protocol for PKA C-alpha specific antibody 55388-1-APDownload protocol
IP protocol for PKA C-alpha specific antibody 55388-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Med Rep

PMID: 30365072

Authors - Zhenyu Qiu
mouseWB

Neural Plast

Neural Plasticity Associated with Hippocampal PKA-CREB and NMDA Signaling Is Involved in the Antidepressant Effect of Repeated Low Dose of Yueju Pill on Chronic Mouse Model of Learned Helplessness.

Authors - Zhilu Zou

CNS Neurosci Ther

Increased Expression of Transient Receptor Potential Vanilloid 4 in Cortical Lesions of Patients with Focal Cortical Dysplasia.

Authors - Xin Chen
humanWB

Proteomics

Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein Upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.

Authors - Hwee Tong Tan
mouseWB

Phytother Res

Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.

Authors - Lei Wu
mouseWB

Front Pharmacol

An Integrative Pharmacology-Based Strategy to Uncover the Mechanism of Xiong-Pi-Fang in Treating Coronary Heart Disease with Depression.

Authors - Lihong Zhang