PKA C-alpha specific Polyclonal antibody
PKA C-alpha specific Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 55388-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human testis tissue, human liver tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human colon cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 9 publications below |
Product Information
55388-1-AP targets PKA C-alpha specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | protein kinase, cAMP-dependent, catalytic, alpha |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 38-43 kDa |
GenBank accession number | NM_002730 |
Gene symbol | PRKACA |
Gene ID (NCBI) | 5566 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus. This antibody is specific to PRKACA.
Protocols
Product Specific Protocols | |
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WB protocol for PKA C-alpha specific antibody 55388-1-AP | Download protocol |
IHC protocol for PKA C-alpha specific antibody 55388-1-AP | Download protocol |
IP protocol for PKA C-alpha specific antibody 55388-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization. | ||
Signal Transduct Target Ther Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma. | ||
Evid Based Complement Alternat Med Repeated Yueju, But Not Fluoxetine, Induced Sustained Antidepressant Activity in a Mouse Model of Chronic Learned Helplessness: Involvement of CaMKII Signaling in the Hippocampus. | ||
PLoS One Quantitative proteomic analysis identified differentially expressed proteins with tail/rump fat deposition in Chinese thin- and fat-tailed lambs. | ||
Neural Plast Neural Plasticity Associated with Hippocampal PKA-CREB and NMDA Signaling Is Involved in the Antidepressant Effect of Repeated Low Dose of Yueju Pill on Chronic Mouse Model of Learned Helplessness. |