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What's the best dilution for PRKACG in ?
Tested Applications
| Positive WB detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55346-1-AP targets PRKACG in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein kinase, cAMP-dependent, catalytic, gamma |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | NM_002732 |
| Gene Symbol | PRKACG |
| Gene ID (NCBI) | 5568 |
| RRID | AB_2881314 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22612 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRKACG antibody 55346-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRKACG Polyclonal antibody (Cat# 55346-1-AP) has 3 total citations. These appear in Environmental International, Journal of Ethnopharmacology, and Human Cell. The associated research fields include reproductive toxicology (DBDPE-induced spermatogenic disorder), metabolic regulation (obesity and brown fat thermogenesis via microbiota modulation), and liver fibrosis (hepatic stellate cell activation). All three citations used the antibody for Western blot in mouse or human samples.
| Species | Application | Title |
|---|---|---|
Environ Int Molecular mechanisms of DBDPE-induced spermatogenic disorder: A network toxicology, machine learning, and in vivo experimental validation. | ||
J Ethnopharmacol Flavonoid-rich extracts of Nelumbo nucifera leaves alleviate obesity in HFD-fed mice via microbiota-dependent modulation of brown fat thermogenesis | ||
Hum Cell The miR-3074/BMP7 axis regulates TGF-β-caused activation of hepatic stellate cells in vitro and CCl4-caused murine liver fibrosis in vivo |
