AMPK Gamma 1 Polyclonal antibody

AMPK Gamma 1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10290-1-AP

Synonyms

AMPK gamma1, AMPK subunit gamma 1, AMPKG, PRKAG1



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10290-1-AP targets AMPK Gamma 1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Gamma 1 fusion protein Ag0302
Full Name protein kinase, AMP-activated, gamma 1 non-catalytic subunit
Calculated molecular weight 38 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC000358
Gene symbol PRKAG1
Gene ID (NCBI) 5571
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1, synonyms: AMPKG, MGC8666) is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an catalytic subunit, and non-catalytic and subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and iAMP-activated protein kinase (AMPK) is a highly conserved heterotrimeric serine/threonine kinase widely characterised as a sensor of cellular energetic stress. AMPK is a heterotrimeric complex consisting of a catalytic α-subunit and two regulatory subunits (β and γ). AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma 1 is one of the gamma regulatory subunits of AMPK.

Protocols

Product Specific Protocols
WB protocol for AMPK Gamma 1 antibody 10290-1-APDownload protocol
IHC protocol for AMPK Gamma 1 antibody 10290-1-APDownload protocol
IF protocol for AMPK Gamma 1 antibody 10290-1-APDownload protocol
IP protocol for AMPK Gamma 1 antibody 10290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Protein Cell

AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth.

Authors - Zhu Kun K