PRKAR1A Polyclonal antibody, PBS Only

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• PRKAR1A Polyclonal antibody, PBS Only (20358-1-PBS)

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  WB analysis using 20358-1-AP (same clone as 20358-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20358-1-AP (PRKAR1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKAR1A antibody (20358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

PRKAR1A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20358-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PPNAD1, PKR1, CNC1, CNC, CAR

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Product Information

20358-1-PBS targets PRKAR1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14216

  Product name: Recombinant human PRKAR1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-100 aa of BC036285

  Sequence: MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAI

• Full Name: protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
• Calculated Molecular Weight: 381 aa, 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC036285
• Gene Symbol: PRKAR1A
• Gene ID (NCBI): 5573
• RRID: AB_10697659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10644
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.