PRKAR1B Polyclonal antibody

PRKAR1B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17991-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

cAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHC

WB analysis of mouse brain using 17991-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 17991-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Transfected HEK-293 using 17991-1-AP
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 17991-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for PRKAR1B in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, Transfected HEK-293 cells, HeLa cells
Positive IHC detected in	human breast cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17991-1-AP targets PRKAR1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12426 Product name: Recombinant human PRKAR1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-381 aa of BC026734 Sequence: MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAHLDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKLDRPRFERVLGPCSEILKRNIQRYNSFISLTV Predict reactive species
Full Name	protein kinase, cAMP-dependent, regulatory, type I, beta
Calculated Molecular Weight	381 aa, 43 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC026734
Gene Symbol	PRKAR1B
Gene ID (NCBI)	5575
RRID	AB_2878478
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31321
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for PRKAR1B antibody 17991-1-AP	Download protocol
WB protocol for PRKAR1B antibody 17991-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PRKAR1B Polyclonal antibody (Cat# 17991-1-AP) has 1 total citation. It was cited in Frontiers in Immunology (Impact Factor: 7), in a 2026 study investigating PRKAR1B as an oncogenic biomarker for diagnostic and prognostic stratification of tumor immunity, proliferation, and migration in head and neck squamous cell carcinoma. The research field is tumor immunology and head and neck cancer. The antibody was used for WB and IF applications on human samples.

Species	Application	Title
human	WB,IF	Front Immunol PRKAR1B as an oncogenic biomarker for diagnostic and prognostic stratification of tumor immunity, proliferation, and migration in head and neck squamous cell carcinoma. Authors - Peng Zhao KD Validated View Article