Tested Applications
| Positive WB detected in | HeLa cells, human testis tissue, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells |
| Positive IP detected in | HeLa cells, HeLa cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10142-2-AP targets PRKAR2A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0203 Product name: Recombinant human PRKAR2A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 74-382 aa of BC002763 Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ Predict reactive species |
| Full Name | protein kinase, cAMP-dependent, regulatory, type II, alpha |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 46-52 kDa |
| GenBank Accession Number | BC002763 |
| Gene Symbol | PRKAR2A |
| Gene ID (NCBI) | 5576 |
| RRID | AB_2168236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13861 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRKAR2A antibody 10142-2-AP | Download protocol |
| IHC protocol for PRKAR2A antibody 10142-2-AP | Download protocol |
| IP protocol for PRKAR2A antibody 10142-2-AP | Download protocol |
| WB protocol for PRKAR2A antibody 10142-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
10142-2-AP (anti-PRKAR1A) has 7 citations. Journals include Signal Transduction and Targeted Therapy, Cancer Letters, Journal of Translational Medicine, Cell Reports, Life Sciences, and Hereditas. Research fields span alcohol-associated liver disease, esophageal squamous cell carcinoma, Parkinson's disease, renal cell carcinoma, PKA signaling regulation, traumatic brain injury, and myocardial infarction. Applications were primarily WB, with some IP and IHC.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease | ||
Cancer Lett Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma.
| ||
J Transl Med F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration | ||
J Transl Med Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease | ||
Life Sci TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats. |
