PRKAR2A Polyclonal antibody

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  WB analysis using 10142-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10142-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10142-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10142-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10142-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10142-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10142-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 10142-2-AP

  IP result of anti-PRKAR2A (10142-2-AP for IP and Detection).

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  IP experiment of HeLa using 10142-2-AP

  IP result of anti-PRKAR2A (IP:10142-2-AP, 4ug; Detection:10142-2-AP 1:2000) with HeLa cells lysate 1320 ug.

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  IHC staining of mouse colon using 10142-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10142-2-AP (PRKAR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 10142-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRKAR2A antibody (10142-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PRKAR2A Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 10142-2-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

cAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells, human testis tissue
• Positive IP detected in: HeLa cells, HeLa cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

10142-2-AP targets PRKAR2A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0203

  Product name: Recombinant human PRKAR2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 74-382 aa of BC002763

  Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ

• Full Name: protein kinase, cAMP-dependent, regulatory, type II, alpha
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 46-52 kDa
• GenBank Accession Number: BC002763
• Gene Symbol: PRKAR2A
• Gene ID (NCBI): 5576
• RRID: AB_2168236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13861
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease; Authors - Chandana Thimme Gowda
human	WB	Cancer Lett; Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma.; Authors - Furong Huang ; KD Validated
rat	WB,IHC	Life Sci; TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats.; Authors - Haochen Wang
mouse,human	WB,IP	Cell Rep; STK25 inhibits PKA signaling by phosphorylating PRKAR1A; Authors - Xiaokan Zhang
mouse	WB	J Transl Med; Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease; Authors - Qinglong Guo
human	WB	J Transl Med; F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration; Authors - Wuping Yang