- Featured Product
- KD/KO Validated
PRKCSH Polyclonal antibody
PRKCSH Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12148-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
12148-1-AP targets PRKCSH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PRKCSH fusion protein Ag2796 |
Full Name | protein kinase C substrate 80K-H |
Calculated molecular weight | 80 kDa |
Observed molecular weight | 80 kDa |
GenBank accession number | BC013586 |
Gene symbol | PRKCSH |
Gene ID (NCBI) | 5589 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PRKCSH antibody 12148-1-AP | Download protocol |
IHC protocol for PRKCSH antibody 12148-1-AP | Download protocol |
IF protocol for PRKCSH antibody 12148-1-AP | Download protocol |
IP protocol for PRKCSH antibody 12148-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway.
| ||
Exp Physiol AGEs-RAGE axis mediates myocardial fibrosis via activation of cardiac fibroblasts induced by autophagy in heart failure. | ||
Cell Rep Spatial region-resolved proteome map reveals mechanism of COVID-19-associated heart injury. | ||
Biochim Biophys Acta Hepatocystin/80K-H inhibits replication of hepatitis B virus through interaction with HBx protein in hepatoma cell. | ||
Mol Cell Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation. |