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PRKCSH Polyclonal antibody

PRKCSH Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12148-1-AP

Synonyms

80K H protein, AGE R2, G19P1, Glucosidase 2 subunit beta, Glucosidase II subunit beta, PCLD, PKCSH, PLD1, PRKCSH



Tested Applications

Positive WB detected inA431 cells, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12148-1-AP targets PRKCSH in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKCSH fusion protein Ag2796
Full Name protein kinase C substrate 80K-H
Calculated molecular weight 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC013586
Gene symbol PRKCSH
Gene ID (NCBI) 5589
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.

Protocols

Product Specific Protocols
WB protocol for PRKCSH antibody 12148-1-APDownload protocol
IHC protocol for PRKCSH antibody 12148-1-APDownload protocol
IF protocol for PRKCSH antibody 12148-1-APDownload protocol
IP protocol for PRKCSH antibody 12148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Mol Cell

Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation.

Authors - Dara E Leto
humanIF

Biochim Biophys Acta

Hepatocystin/80K-H inhibits replication of hepatitis B virus through interaction with HBx protein in hepatoma cell.

Authors - Shin Gu-Choul GC
humanWB

Nat Commun

PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway.

Authors - Gu-Choul Shin
  • KD Validated