Tested Applications
| Positive WB detected in | A431 cells, mouse liver tissue, rat liver tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12148-1-AP targets PRKCSH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2796 Product name: Recombinant human PRKCSH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 157-506 aa of BC013586 Sequence: TVKEEAEKPEREAKEQHQKLWEEQLAAAKAQQEQELAADAFKELDDDMDGTVSVTELQTHPELDTDGDGALSEAEAQALLSGDTQTDATSFYDRVWAAIRDKYRSEALPTDLPTPSAPDLTEPKEEQPPVPSSPTEEEEEEEEEEEEAEEEEEEEDSEEAPPPLSPPQPASPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEFAYLYSQCYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHDEL Predict reactive species |
| Full Name | protein kinase C substrate 80K-H |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC013586 |
| Gene Symbol | PRKCSH |
| Gene ID (NCBI) | 5589 |
| RRID | AB_2253107 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14314 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRKCSH antibody 12148-1-AP | Download protocol |
| IHC protocol for PRKCSH antibody 12148-1-AP | Download protocol |
| IP protocol for PRKCSH antibody 12148-1-AP | Download protocol |
| WB protocol for PRKCSH antibody 12148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PRKCSH Polyclonal antibody (Cat# 12148-1-AP) has 13 citations published in journals including Nature Biotechnology, Nature Communications, Bone Research, Experimental & Molecular Medicine, Molecular Cell, Cell Reports, Metabolic Engineering, JCI Insight, Journal of Cell Biology, European Journal of Medical Research, Experimental Physiology, BBA, and Heliyon. Research fields span gene editing, mechanobiology, cancer biology, ER-associated degradation, cardiovascular disease, glycoprotein secretion, protein quality control, viral hepatitis, and pulmonary fibrosis.
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Donor-complementary prime editing enables precise kilobase and library-compatible DNA insertions | ||
Bone Res Matrix stiffness regulates nucleus pulposus cell glycolysis by MRTF-A-dependent mechanotransduction | ||
Nat Commun PRKCSH contributes to tumorigenesis by selective boosting of IRE1 signaling pathway.
| ||
Exp Mol Med PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer | ||
Mol Cell Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation. | ||
Cell Rep Spatial region-resolved proteome map reveals mechanism of COVID-19-associated heart injury. |
