PRKD1 Polyclonal antibody

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  WB analysis using 20714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20714-1-AP (PRKD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20714-1-AP (PRKD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 20714-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 20714-1-AP (PRKD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 20714-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 20714-1-AP (PRKD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRKD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20714-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

PKD, PKD1, PRKCM, Serine/threonine protein kinase D1

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Tested Applications

• Positive WB detected in: LNCaP cells, DU 145 cells, HEK-293 cells
• Positive IHC detected in: rat cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

20714-1-AP targets PRKD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase D1
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: NM_002742
• Gene Symbol: PRKD1
• Gene ID (NCBI): 5587
• RRID: AB_3669341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15139
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Adv Sci (Weinh); Single-Cell Analysis Clarifies Pathological Heterogeneity in Tenosynovial Giant Cell Tumor and Identifies Biomarkers for Predicting Disease Recurrence; Authors - Yubin Xie