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  • KD/KO Validated

PRKD2 Polyclonal antibody

PRKD2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11623-1-AP

Synonyms

HSPC187, PKD2, PRKD2, protein kinase D2



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells, human kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11623-1-AP targets PRKD2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKD2 fusion protein Ag2192
Full Name protein kinase D2
Calculated molecular weight 878 aa, 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC025307
Gene symbol PRKD2
Gene ID (NCBI) 25865
RRIDAB_2172676
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRKD2 antibody 11623-1-APDownload protocol
IHC protocol for PRKD2 antibody 11623-1-APDownload protocol
IP protocol for PRKD2 antibody 11623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Life Sci

The novel mechanism of human norovirus induced diarrhea: Activation of PKD2 caused by HuNoVs destroyed AQP3 expression through AP2γ in intestinal epithelial cells

Authors - Ling Huang
  • KD Validated
mouseWB

J Cell Sci

Characterization of lipoprotein lipase storage vesicles in 3T3-L1 adipocytes.

Authors - Benjamin Roberts
zebrafishWB,IF

J Physiol

Regulation of the PKD2 channel function by TACAN

Authors - Xiong Liu
  • KD Validated