PRKD3 Polyclonal antibody

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• PRKD3 Polyclonal antibody (12785-1-AP)

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  WB analysis using 12785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 12785-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12785-1-AP

  IP result of anti-PRKD3 (IP:12785-1-AP, 4ug; Detection:12785-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human breast cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of DU 145 using 12785-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed DU 145 cells using PRKD3 antibody (12785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PRKD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12785-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.7.11.13, EPK2, nPKC-nu, PKD3, PRKCN

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, NIH/3T3 cells, C2C12 cells, Raji cells, Ramos cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12785-1-AP targets PRKD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3499

  Product name: Recombinant human PRKD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-345 aa of BC030706

  Sequence: MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSTDEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDID

• Full Name: protein kinase D3
• Calculated Molecular Weight: 611aa,68 kDa; 100 kDa; 890aa
• Observed Molecular Weight: 68 kDa, 100 kDa
• GenBank Accession Number: BC030706
• Gene Symbol: PRKD3
• Gene ID (NCBI): 23683
• RRID: AB_2877881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94806
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein kinase D 3 (PRKD3), a serine/threonine protein kinase, functions as a crucial regulator across numerous cancer types. High mRNA and protein levels of PRKD3 were reported to be associated with multiple tumor nodules, vascular infiltration, advanced AJCC staging, and poor prognosis in patients with hepatocellular carcinoma (HCC) after liver resection, highlighting the oncogenic function of PRKD3 (PMID: 39678603, 41980898).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,IF	Exp Mol Med; Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling; Authors - Chunlei Liu
human	WB	NAR Cancer; Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs; Authors - Spencer Arnesen
human	IHC	Cancer Rep (Hoboken); PRKD3 Overexpression May Improve Survival and Suppresses Proliferation in Colorectal Cancer.; Authors - Bin Zhang