DNA-PKcs Recombinant antibody, PBS Only

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  WB analysis of HT-29 using 85370-2-RR (same clone as 85370-2-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 85370-2-RR (DNA-PKcs antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 85370-2-RR (same clone as 85370-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 85370-2-RR (DNA-PKcs antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85370-2-RR (same clone as 85370-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85370-2-RR (DNA-PKcs antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85370-2-RR (same clone as 85370-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85370-2-RR (DNA-PKcs antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 85370-2-RR (same clone as 85370-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DNA-PKcs antibody (85370-2-RR, Clone: 242676E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85370-2-RR (same clone as 85370-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA-PKcs antibody (85370-2-RR, Clone: 242676E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85370-2-RR (same clone as 85370-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNA-PKcs antibody (85370-2-RR, Clone: 242676E6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 85370-2-RR (same clone as 85370-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DNA-PKcs Recombinant antibody (85370-2-RR, Clone:242676E6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85370-2-PBS in a different storage buffer formulation.

DNA-PKcs Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 85370-2-PBS

Clone No.242676E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

DNAPK, DNPK1, PRKDC, DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit

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Product Information

85370-2-PBS targets DNA-PKcs in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase, DNA-activated, catalytic polypeptide
• Calculated Molecular Weight: 469 kDa
• Observed Molecular Weight: 350-460 kDa
• GenBank Accession Number: NM_006904
• Gene Symbol: PRKDC
• Gene ID (NCBI): 5591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78527
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism.