Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, C2C12 cells, PC-12 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10771-1-AP targets PACT in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1214 Product name: Recombinant human PRKRA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC009470 Sequence: MSQSRHRAEAPPLEREDSGTFSLGKMITAKPGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRAAEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGPAHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCTWHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAELSTSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERK Predict reactive species |
| Full Name | protein kinase, interferon-inducible double stranded RNA dependent activator |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 31-34 kDa |
| GenBank Accession Number | BC009470 |
| Gene Symbol | PRKRA |
| Gene ID (NCBI) | 8575 |
| RRID | AB_2170496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75569 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PACT antibody 10771-1-AP | Download protocol |
| WB protocol for PACT antibody 10771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PACT (PRKRA) Polyclonal antibody, catalog number 10771-1-AP, has 16 citations. These appear in journals including Molecular Cell, Nucleic Acids Research, Acta Pharmaceutica Sinica B, Science Advances, Cell Reports, EMBO Reports, PLoS Pathogens, Journal of Neurochemistry, Journal of Biological Chemistry, Heliyon, Journal of Proteome Research, Disease Models & Mechanisms, Molecular Biology of the Cell, Translational Cancer Research, and bioRxiv. Research fields span innate immunity and antiviral pathways, RNA biology, cancer, mitochondrial biogenesis, and neurology.
| Species | Application | Title |
|---|---|---|
Mol Cell Prkra dimer senses double-stranded RNAs to dictate global translation efficiency | ||
Nucleic Acids Res Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA. | ||
Acta Pharm Sin B A new approach to discovering a broad-spectrum anti-respiratory virus drug: Tubeimoside II modulates host factor PACT to potentiate the RIG-I antiviral pathway.
| ||
Sci Adv Biomimetic targeted self-adaptive nanodrug for inflammation optimization and AT2 cell modulation in precise ARDS therapy | ||
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
Cell Rep Virus-induced lncRNA-BTX allows viral replication by regulating intracellular translocation of DHX9 and ILF3 to induce innate escape |
Reviews
What customers say
The product received a 4-star rating from a user who tested it on human fibroblasts by Western Blot at a 1:1000 dilution. The reviewer reported a single band of the expected size, indicating good specificity and performance for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hannah (Verified Customer) (02-18-2025) | single band of expected size
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