PRMT1 Monoclonal antibody, PBS Only

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  WB analysis using 68365-1-Ig (same clone as 68365-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68365-1-Ig (PRMT1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68365-1-Ig (same clone as 68365-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (68365-1-Ig, Clone: 1G8E2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68365-1-Ig (same clone as 68365-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 ug PRMT1 Monoclonal antibody (68365-1-Ig, Clone:1G8E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68365-1-PBS in a different storage buffer formulation.

PRMT1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 68365-1-PBS

Clone No.1G8E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1

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Product Information

68365-1-PBS targets PRMT1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29063

  Product name: Recombinant human PRMT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-343 aa of BC019268

  Sequence: MEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR

• Full Name: protein arginine methyltransferase 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38~42 kDa
• GenBank Accession Number: BC019268
• Gene Symbol: PRMT1
• Gene ID (NCBI): 3276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99873
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.