PRMT5 Recombinant antibody, PBS Only

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  WB analysis using 82750-2-RR (same clone as 82750-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82750-2-RR (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 82750-2-RR (same clone as 82750-2-PBS)

  IP result of anti-PRMT5 (IP:82750-2-RR, 4ug; Detection:82750-2-RR 1:3000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82750-2-RR (same clone as 82750-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (82750-2-RR, Clone: 250553B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82750-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82750-2-RR against Human PRMT5 were performed. The affinity constant is 0.923 nM.

PRMT5 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 82750-2-PBS

Clone No.250553B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

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Product Information

82750-2-PBS targets PRMT5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRMT5 fusion protein Ag9736
• Full Name: protein arginine methyltransferase 5
• Calculated Molecular Weight: 637 aa, 73 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC025979
• Gene Symbol: PRMT5
• Gene ID (NCBI): 10419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14744
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.