PRMT6 Polyclonal antibody

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• PRMT6 Polyclonal antibody (15395-1-AP)

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• KD/KO Validated

PRMT6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15395-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

EC:2.1.1.319, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 6, Histone-arginine N-methyltransferase PRMT6, HRMT1L6, PRMT 6

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  WB analysis using 15395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of mouse brain using 15395-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HEK-293 using 15395-1-AP

  IP result of anti-PRMT6 (IP:15395-1-AP, 4ug; Detection:15395-1-AP 1:1500) with HEK-293 cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human prostate cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human prostate cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human placenta using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human stomach cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of mouse stomach using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human kidney using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human kidney using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human testis using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human testis using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15395-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IF Staining of MCF-7 using 15395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PRMT6 antibody (15395-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of MCF-7 using 15395-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug PRMT6 Polyclonal antibody (15395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue, human kidney tissue, human breast cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15395-1-AP targets PRMT6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7592

  Product name: Recombinant human PRMT6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-316 aa of BC002729

  Sequence: MIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED

• Full Name: protein arginine methyltransferase 6
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC002729
• Gene Symbol: PRMT6
• Gene ID (NCBI): 55170
• RRID: AB_2237723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96LA8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT6 belongs to the protein arginine methyltransferase (PRMT) family that catalyzes the mono- and dimethylation of arginine residues in a variety of proteins. PRMT6 is a nuclear protein and is predominantly expressed in testis and kidney. Western blot analysis using this antibody detected a major band around 42 kDa in HeLa cells.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	EMBO Rep; Arginine methylation of SIRT7 couples glucose sensing with mitochondria biogenesis.; Authors - Wei-Wei Yan ; KD Validated
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu
mouse	WB	Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
mouse	IHC	Aging (Albany NY); Cigarette smoke extract induces airway epithelial cell death via repressing PRMT6/AKT signaling.; Authors - Tiao Li
human	WB,IHC	Int J Oncol; Inhibition of protein arginine methyltransferase 6 activates interferon signaling and induces the apoptosis of endometrial cancer cells via histone modification; Authors - Futaba Inoue
human	WB	Front Cell Dev Biol; F-Box Protein FBXW17-Mediated Proteasomal Degradation of Protein Methyltransferase PRMT6 Exaggerates CSE-Induced Lung Epithelial Inflammation and Apoptosis.; Authors - Tiao Li