PRMT7 Polyclonal antibody

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  WB analysis using 21688-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21688-1-AP (PRMT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21688-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21688-1-AP (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21688-1-AP

  IP result of anti-PRMT7 (IP:21688-1-AP, 4ug; Detection:21688-1-AP 1:1000) with HeLa cells lysate 1400 ug.

PRMT7 Polyclonal Antibody for WB, IP, ELISA

Cat No. 21688-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Protein arginine N-methyltransferase 7, PRMT 7, Histone-arginine N-methyltransferase PRMT7, EC:2.1.1.321, [Myelin basic protein]-arginine N-methyltransferase PRMT7

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21688-1-AP targets PRMT7 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRMT7 fusion protein Ag16269
• Full Name: protein arginine methyltransferase 7
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 73-78 kDa
• GenBank Accession Number: BC000146
• Gene Symbol: PRMT7
• Gene ID (NCBI): 54496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NVM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.