Tested Applications
| Positive WB detected in | Y79 cells |
| Positive IHC detected in | rat brain tissue, human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12557-1-AP targets PRMT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3166 Product name: Recombinant human PRMT8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 95-394 aa of BC022458 Sequence: RTLTYRNSMYHNKHVFKDKVVLDVGSGTGILSMFAAKAGAKKVFGIECSSISDYSQKIIKANHLDNIITIFKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIFARDKWLKPGGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDIYTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRRGEEIYGTISMKPNAKNVRDLDFTVDLDFKGQLCETSVSNDYKMR Predict reactive species |
| Full Name | protein arginine methyltransferase 8 |
| Calculated Molecular Weight | 394 aa, 45 kDa |
| Observed Molecular Weight | 43-50 kDa |
| GenBank Accession Number | BC022458 |
| Gene Symbol | PRMT8 |
| Gene ID (NCBI) | 56341 |
| RRID | AB_2171964 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR22 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRMT8 is a member of arginine methyl transferase (PRMT) family which acts as nuclear receptor coactivators. PRMT8 catalyzes the asymmetric arginine methylation and is involved in cellular differentiation. PRMT8 has several isoforms; the full-length isoform has a brain specific expression pattern and is localized to the plasma membrane; the truncated isoforms display nuclear localization and are expressed more widely. This antibody recognizes all of isoforms of PRMT8.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PRMT8 antibody 12557-1-AP | Download protocol |
| WB protocol for PRMT8 antibody 12557-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-PRMT1 antibody (Cat# 12557-1-AP) has 4 total citations, all applied in Western Blot. Citations appear in Journal of Medicinal Chemistry, Biochemical Pharmacology, Toxicology and Applied Pharmacology, and Basic & Clinical Pharmacology and Toxicology. Associated research fields include PROTAC degrader discovery, acute myeloid leukemia (MYC-PRMT pathway), hepatocellular carcinoma, and soft tissue sarcoma.
| Species | Application | Title |
|---|---|---|
J Med Chem Discovery of the First-in-Class Protein Arginine Methyltransferase 1 PROTAC Degrader. | ||
Biochem Pharmacol A novel 3-alkenylindol-2-one derivative selectively inhibits the growth of acute myeloid leukemia via modulation of the MYC-PRMT pathway | ||
Toxicol Appl Pharmacol Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5 | ||
Basic Clin Pharmacol Toxicol Ribavirin inhibits cell proliferation and metastasis and prolongs survival in soft tissue sarcomas by downregulating both protein arginine methyltransferases 1 and 5. |
