CD133-1,2,4,7 Polyclonal antibody

CD133-1,2,4,7 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19945-1-AP

Synonyms

AC133, Antigen AC133, CD133, CD133-1,2,4,7, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4



Tested Applications

Positive WB detected inY79 cells, mouse lung tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19945-1-AP targets CD133-1,2,4,7 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight 115 kDa
GenBank accession numberNM_001145851
Gene symbol PROM1
Gene ID (NCBI) 8842
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2,4,7 (UNIPROT has updated the info of isoforms)of CD133.

Protocols

Product Specific Protocols
WB protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IHC protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IP protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols