PROP1 Polyclonal antibody

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• PROP1 Polyclonal antibody (18739-1-AP)

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  WB analysis using 18739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18739-1-AP (PROP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 18739-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18739-1-AP (PROP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18739-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18739-1-AP (PROP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PROP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18739-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Homeobox protein prophet of Pit-1, Pituitary-specific homeodomain factor, PROP 1, PROP paired like homeobox 1, PROP-1

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse embryo
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18739-1-AP targets PROP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: PROP paired-like homeobox 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC069076
• Gene Symbol: PROP1
• Gene ID (NCBI): 5626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75360
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PROP1 belongs to the paired homeobox family. It contains one homeobox DNA-binding domain. PROP1 is possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes. It is expressed specifically in embryonic pituitary. Mutation of PROP1 will cause combined pituitary hormone deficiency (CPHD) which is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.