PROX1 Monoclonal antibody

PROX1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D4B1

Cat no : 67438-1-Ig

Synonyms

prospero homeobox 1, Prospero homeobox protein 1, PROX 1, PROX1



Tested Applications

Positive WB detected inrat liver tissue, HuH-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

67438-1-Ig targets PROX1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PROX1 fusion protein Ag9681
Full Name prospero homeobox 1
Calculated molecular weight 737 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC024201
Gene symbol PROX1
Gene ID (NCBI) 5629
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

Protocols

Product Specific Protocols
WB protocol for PROX1 antibody 67438-1-IgDownload protocol
IHC protocol for PROX1 antibody 67438-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols