PRPF19 Recombinant monoclonal antibody

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• PRPF19 Recombinant monoclonal antibody (88331-1-RR)

PRPF19 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 88331-1-RR

Clone No.260837A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.3.2.27, hPso4, Pre-mRNA-processing factor 19, PRP19, PSO4

• 

  WB analysis using 88331-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88331-1-RR (PRPF19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 88331-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRPF19 antibody (88331-1-RR, Clone: 260837A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, PC-3 cells, Jurkat cells, A431 cells, mouse kidnet tissue, rat kidney tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88331-1-RR targets PRPF19 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7700

  Product name: Recombinant human PRPF19 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 155-504 aa of BC008719

  Sequence: GAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL

• Full Name: PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008719
• Gene Symbol: PRPF19
• Gene ID (NCBI): 27339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UMS4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.