PRPF31 Polyclonal antibody, PBS Only

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• PRPF31 Polyclonal antibody, PBS Only (27750-1-PBS)

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  WB analysis using 27750-1-AP (same clone as 27750-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27750-1-AP (PRPF31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 27750-1-AP (same clone as 27750-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27750-1-PBS in a different storage buffer formulation.

PRPF31 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 27750-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

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Product Information

27750-1-PBS targets PRPF31 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26716

  Product name: Recombinant human PRPF31 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 101-212 aa of NM_015629

  Sequence: MVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRM

• Full Name: PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_015629
• Gene Symbol: PRPF31
• Gene ID (NCBI): 26121
• RRID: AB_2918130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWY3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.