PRPF38B Polyclonal antibody

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  WB analysis using 23901-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23901-1-AP (PRPF38B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PRPF38B Polyclonal Antibody for WB, ELISA

Cat No. 23901-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse

Applications

WB, ELISA

Synonyms

Pre mRNA splicing factor 38B, PRPF38B, RP11 293A10.1, Sarcoma antigen NY SAR 27

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23901-1-AP targets PRPF38B in WB, ELISA applications and shows reactivity with human, Mouse samples.

• Tested Reactivity: human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20983

  Product name: Recombinant human PRPF38B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-186 aa of BC130346

  Sequence: MANNSPALTGNSQPQHQAAAAAAQQQQQCGGGGATKPAVSGKQGNVLPLWGNEKTMNLNPMILTNILSSPYFKVQLYELKTYHEVVDEIYFKVTHVEPWEKGSRKTAGQTGMCGGVRGVGTGGIVSTAFCLLYKLFTLKLTRKQVMGLITHTDSPYIRALGFMYIRYTQPPTDLWDWFESFLDDEE

• Full Name: PRP38 pre-mRNA processing factor 38 (yeast) domain containing B
• Calculated Molecular Weight: 546 aa, 64 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC130346
• Gene Symbol: PRPF38B
• Gene ID (NCBI): 55119
• RRID: AB_3085715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q5VTL8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF38B (Pre-mRNA Processing Factor 38B) is a unique component of the U4/U6.U5 tri small nuclear ribonuclear protein (snRNP) particle and is necessary for an essential step in late spliceosome maturation.