PRPF40A Polyclonal antibody

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  WB analysis of HEK-293 using 17392-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17392-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17392-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17392-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17392-1-AP

  IP result of anti-PRPF40A (IP:17392-1-AP, 4ug; Detection:17392-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human lung cancer using 17392-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17392-1-AP (PRPF40A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 17392-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17392-1-AP (PRPF40A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRPF40A Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17392-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Fas ligand associated factor 1, Fas ligand-associated factor 1, FBP 11, FBP11, FLAF1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17392-1-AP targets PRPF40A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11290

  Product name: Recombinant human PRPF40A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 743-957 aa of BC027178

  Sequence: MKRKESAFKSMLKQAAPPIELDAVWEDIRERFVKEPAFEDITLESERKRIFKDFMHVLEHECQHHHSKNKKHSKKSKKHHRKRSRSRSGSDSDDDDSHSKKKRQRSESRSASEHSSSAESERSYKKSKKHKKKSKKRRHKSDSPESDAEREKDKKEKDRESEKDRTRQRSESKHKSPKKKTGKDSGNWDTSGSELSEGELEKRRRTLLEQLDDDQ

• Full Name: PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 957 aa, 109 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC027178
• Gene Symbol: PRPF40A
• Gene ID (NCBI): 55660
• RRID: AB_2171031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75400
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.