PRPH2 Monoclonal antibody, PBS Only (Capture)

• 

  WB analysis using 68780-1-Ig (same clone as 68780-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68780-1-Ig (PRPH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68780-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68780-1-Ig (same clone as 68780-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68780-1-Ig (PRPH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68780-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50146-1

  Cytometric bead array standard curve of MP50146-1, PRPH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68780-1-PBS. Detection antibody: 68780-2-PBS. Standard:Ag12555. Range: 1.563-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 68780-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 68780-1-Ig against Human PRPH2 was performed. The affinity constant is below 1 pM.

PRPH2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 68780-1-PBS

Clone No.2F11A1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

2F11A1, AOFMD, AVMD, Peripherin 2, Peripherin2

---

Product Information

68780-1-PBS targets PRPH2 as part of a matched antibody pair:

MP50146-1: 68780-1-PBS capture and 68780-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12555

  Product name: Recombinant human PRPH2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 124-346 aa of BC074720

  Sequence: GSLENTLGQGLKNGMKYYRDTDTPGRCFMKKTIDMLQIEFKCCGNNGFRDWFEIQWISNRYLDFSSKEVKDRIKSNVDGRYLVDGVPFSCCNPSSPRPCIQYQITNNSAHYSYDHQTEELNLWVRGCRAALLSYYSSLMNSMGVVTLLIWLFEVTITIGLRYLQTSLDGVSNPEESESESEGWLLEKSVPETWKAFLESVKKLGKGNQVEAEGAGAGQAPEAG

• Full Name: peripherin 2 (retinal degeneration, slow)
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 35-39 kDa
• GenBank Accession Number: BC074720
• Gene Symbol: PRPH2
• Gene ID (NCBI): 5961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23942
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).