PRPS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRPS2 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27024-1-AP

Synonyms

PPRibP, PRPS2, PRS II, PRSII

---

Validation Data Gallery

• 

  WB analysis of A375 using 27024-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27024-1-AP (PRPS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 27024-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27024-1-AP (PRPS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 27024-1-AP

  IP result of anti-PRPS2 (IP:27024-1-AP, 4ug; Detection:27024-1-AP 1:1000) with mouse spleen tissue lysate 1560 ug.

• 

  IP experiment of A375 using 27024-1-AP

  IP result of anti-PRPS2 (IP:27024-1-AP, 4ug; Detection:27024-1-AP 1:1000) with A375 cells lysate 1600 ug.

• 

  IF Staining of A375 using 27024-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using PRPS2 antibody (27024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: A375 cells
• Positive IP detected in: A375 cells, mouse spleen tissue
• Positive IF detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Product Information

27024-1-AP targets PRPS2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPS2 fusion protein Ag25785
• Full Name: phosphoribosyl pyrophosphate synthetase 2
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC119662
• Gene Symbol: PRPS2
• Gene ID (NCBI): 5634
• RRID: AB_2880726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPS (phosphoribosyl pyrophosphate synthetase) proteins catalyze the synthesis of phosphoribosyl pyrophosphate (PRPP). Three human PRPS isoforms exist and are encoded by three different genes. PRPS1 and PRPS2 (also known as PRS1 and PRS2, respectively) are ubiquitously expressed, while PRPS3 (also known as PRPS1L1) is specific to the testis. PRPP is an important substrate synthesized from MgATP and ribose-5-phosphate in a reaction that requires inorganic phosphate and magnesium as a cofactor. PRPP is essential in the synthesis of nearly all nucleotides, implying that PRPS proteins play an important role in nucleotide biosynthesis and purine metabolism. PRPS2 is a 318 amino acid protein that exists as a homodimer and a hexamer composed of three homodimers.

Publications

Species	Application	Title
human	CoIP,WB	Sci Adv; NOTCH1-driven UBR7 stimulates nucleotide biosynthesis to promote T cell acute lymphoblastic leukemia.; Authors - Shashank Srivastava
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv ; KD Validated
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran
human	WB	Nat Commun; Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress; Authors - Ronghui Yang ; KD Validated