PRR14 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRR14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22819-1-AP

Synonyms

Proline-rich protein 14, Proline rich protein 14, proline rich 14

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Validation Data Gallery

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  WB analysis using 22819-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 22819-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22819-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22819-1-AP (PRR14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 22819-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 22819-1-AP (PRR14 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 22819-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 22819-1-AP (PRR14 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HCT 116 cells, HL-60 cells, MCF-7 cells, SW480 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

22819-1-AP targets PRR14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRR14 fusion protein Ag14119
• Full Name: proline rich 14
• Calculated Molecular Weight: 585 aa, 64 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC000119
• Gene Symbol: PRR14
• Gene ID (NCBI): 78994
• RRID: AB_2879174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRR14 (proline-rich protein 14) is a 585-amino acid protein with a calculated molecular weight of 64 kDa. This protein functions to tether heterochromatin to the nuclear periphery during interphase, through associations with heterochromatin protein 1 (HP1) and the nuclear lamina. After disassembly early in mitosis, PRR14 reassembles in two steps, first binding to anaphase chromosomes through HP1, followed by association with the nuclear lamina in telophase. PRR14 may thereby play a role in specifying HP1-bound heterochromatin for reattachment to the nuclear lamina at mitotic exit. (PMID: 24209742; 24637393)

Publications

Species	Application	Title
mouse	WB,IF	Nucleus; PRR14 organizes H3K9me3-modified heterochromatin at the nuclear lamina; Authors - Anna A Kiseleva ; KD Validated