Tested Applications
| Positive WB detected in | human kidney tissue, human brain tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue, mouse kidney tissue, human kidney tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17948-1-AP targets PRR5/Protor-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12424 Product name: Recombinant human PRR5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC016921 Sequence: MAPMPTGQKLLDSLAETWDFFFSDVLPMLQAIFYPVQGKEPSVRQLALLHFRNAITLSVKLEDALARAHARVPPAIVQMLLVLQGVHESRGVTEDYLRLETLVQKVVSPYLGTYGLHSSEGPFTHSCILEKRLLRRSRSGDVLAKNPVVRSKSYNTPLLNPVQEHEAEGAAAGGTSIRRHSVSEMTSCPEPQGFSDPPGQGPTGTFRSSPAPHSGPCPSRLYPTTQPPEQGLDPTRSSLPRSSPENLVDQILESVDSDSEGIFIDFGRGRGSGMSDLEGSGGRQSVV Predict reactive species |
| Full Name | proline rich 5 (renal) |
| Calculated Molecular Weight | 31 kDa, 43 kDa |
| Observed Molecular Weight | 33 kDa, 50 kDa |
| GenBank Accession Number | BC016921 |
| Gene Symbol | PRR5 |
| Gene ID (NCBI) | 55615 |
| RRID | AB_2268865 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P85299 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRR5/Protor-1 antibody 17948-1-AP | Download protocol |
| IHC protocol for PRR5/Protor-1 antibody 17948-1-AP | Download protocol |
| IP protocol for PRR5/Protor-1 antibody 17948-1-AP | Download protocol |
| WB protocol for PRR5/Protor-1 antibody 17948-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRR5 antibody (catalog no. 17948-1-AP) has 3 total citations. These appear in Nature Cell Biology, International Journal of Biological Macromolecules, and Science Signaling. The associated research fields include m6A mRNA methylation regulation, endometrial cancer proliferation and tumorigenicity, arsenic carcinogenesis, and LINC01133-to-ERK signaling in triple-negative breast cancer. Applications cited include IHC and WB in human samples.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. | ||
Int J Biol Macromol YTHDF2 promotes arsenic carcinogenesis through m(6)A-dependent SMAD7 decay and PRR5 escape from decay. | ||
Sci Signal Stabilization of IQGAP1 by the mTORC2 component PRR5 mediates mitogenic LINC01133-to-ERK signaling in triple-negative breast cancer. |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used the antibody for Western Blot and Immunofluorescence on human motor neurons and reported that it works very well. The antibody is a rabbit polyclonal targeting FUS/TLS, generated against amino acids 260–274 of mouse FUS/TLS, a region identical to the human sequence. Overall, the feedback is positive, with no issues or concerns raised.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tominica (Verified Customer) (08-19-2019) | Rabbit polyclonal anti-FUS/TLS Ab-TLS-M generated against amino acids 260–274 of mouse FUS/TLS, a stretch where the sequence is identical to the human FUS/TLS sequence. works very well.
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