PRRC1 Polyclonal antibody

PRRC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27887-1-AP

Synonyms

proline rich coiled coil 1, Protein PRRC1, PRRC1

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Validation Data Gallery

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  WB analysis of HeLa using 27887-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27887-1-AP (PRRC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 27887-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27887-1-AP (PRRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 27887-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27887-1-AP (PRRC1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 27887-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 27887-1-AP (PRRC1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27887-1-AP targets PRRC1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRRC1 fusion protein Ag27349
• Full Name: proline-rich coiled-coil 1
• Calculated Molecular Weight: 445 aa, 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC017066
• Gene Symbol: PRRC1
• Gene ID (NCBI): 133619
• RRID: AB_2881005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.