SynDIG4/PRRT1 Polyclonal antibody

SynDIG4/PRRT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17261-1-AP

Synonyms

PRRT1, Proline-rich transmembrane protein 1, NG5, DSPD1, Dispanin subfamily D member 1

---

Validation Data Gallery

• 

  WB analysis of mouse hippocampal tissue using 17261-1-AP

  WB result of PRRT1 antibody (17261-1-AP) with mouse hippocampal tissue lysate. (Image courtesy of Dr. I. Kaur and Dr. E. Diaz, UC Davis).

• 

  WB analysis of mouse brain using 17261-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17261-1-AP (PRRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of transfected cells using 17261-1-AP

  IF result of anti-PRRT1 (17261-1-AP) with COS cell transfected with recombinant HA-PRRT1.

---

Tested Applications

• Positive WB detected in: mouse brain tissue, mouse hippocampal tissue
• Positive IF/ICC detected in: transfected cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

17261-1-AP targets SynDIG4/PRRT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SynDIG4/PRRT1 fusion protein Ag10958
• Full Name: proline-rich transmembrane protein 1
• Calculated Molecular Weight: 225aa,23 kDa; 306aa,31 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC013201
• Gene Symbol: SynDIG4
• Gene ID (NCBI): 80863
• RRID: AB_2878371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SynDIG4 [also known as Prrt1 (proline-rich transmembrane protein 1)] has recently been identified as a component of AMPAR complexes. SynDIG4 is especially prominent in the hippocampus and particularly within CA1.The function of PRRT1 has not been widely studied, and is yet to be fully elucidated.

Publications

Species	Application	Title
rat	WB,IF	Front Synaptic Neurosci; Interaction and Subcellular Association of PRRT1/SynDIG4 With AMPA Receptors.; Authors - Emily Eischen Martin
mouse		Mol Cell Neurosci; PRRT1 regulates basal and plasticity-induced AMPA receptor trafficking.; Authors - Eva Troyano-Rodriguez
	WB	Zoolog Sci; A Calaxin Gene in the Pacific Oyster Crassostrea gigasand Its Potential Roles in Cilia.; Authors - Xiaofei Wang
MOUSE	WB	eNeuro; Loss of SynDIG1 Reduces Excitatory Synapse Maturation But Not Formation In Vivo.; Authors - George Chenaux