PRRX2 Polyclonal antibody

• 

  WB analysis of mouse brain using 23869-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23869-1-AP (PRRX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 23869-1-AP

  IP result of anti-PRRX2 (IP:23869-1-AP, 4ug; Detection:23869-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• Featured Product
• KD/KO Validated

PRRX2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 23869-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

paired related homeobox 2, PMX2, PRRX2, PRX 2, PRX2

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

23869-1-AP targets PRRX2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20917

  Product name: Recombinant human PRRX2 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-253 aa of BC014645

  Sequence: MDSAAAAFALDKPALGPGPPPPPPALGPGDCAQARKNFSVSHLLDLEEVAAAGRLAARPGARAEAREGAAREPSGGSSGSEAAPQDGECPSPGRGSAAKRKKKQRRNRTTFNSSQLQALERVFERTHYPDAFVREELARRVNLSEARVQVWFQNRRAKFRRNERAMLASRSASLLKSYSQEAAIEQPVAPRPTALSPDYLSWTASSPYSTVPPYSPGSSGPATPGVNMANSIASLRLKAKEFSLHHSQVPTVN

• Full Name: paired related homeobox 2
• Calculated Molecular Weight: 253 aa, 27 kDa
• Observed Molecular Weight: 27-34 kDa
• GenBank Accession Number: BC014645
• Gene Symbol: PRRX2
• Gene ID (NCBI): 51450
• RRID: AB_2879344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q99811
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Cell Dev Biol; QSOX2 Is an E2F1 Target Gene and a Novel Serum Biomarker for Monitoring Tumor Growth and Predicting Survival in Advanced NSCLC.; Authors - Yaqi Li
human	WB	Cancer Res; A Genome-Wide CRISPR Activation Screen Identifies PRRX2 as a Regulator of Enzalutamide Resistance in Prostate Cancer; Authors - Yara Rodríguez ; KO Validated
	WB	Redox Biol; A reciprocal relationship between mitochondria and lipid peroxidation determines the chondrocyte intracellular redox environment; Authors - Madeline R Hines