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  • KD/KO Validated

PRRX2 Polyclonal antibody

PRRX2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ChIP, ELISA

paired related homeobox 2, PMX2, PRRX2, PRX 2, PRX2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23869-1-AP targets PRRX2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20917

Product name: Recombinant human PRRX2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-253 aa of BC014645

Sequence: MDSAAAAFALDKPALGPGPPPPPPALGPGDCAQARKNFSVSHLLDLEEVAAAGRLAARPGARAEAREGAAREPSGGSSGSEAAPQDGECPSPGRGSAAKRKKKQRRNRTTFNSSQLQALERVFERTHYPDAFVREELARRVNLSEARVQVWFQNRRAKFRRNERAMLASRSASLLKSYSQEAAIEQPVAPRPTALSPDYLSWTASSPYSTVPPYSPGSSGPATPGVNMANSIASLRLKAKEFSLHHSQVPTVN

Predict reactive species
Full Name paired related homeobox 2
Calculated Molecular Weight 253 aa, 27 kDa
Observed Molecular Weight 27-34 kDa
GenBank Accession NumberBC014645
Gene Symbol PRRX2
Gene ID (NCBI) 51450
RRIDAB_2879344
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ99811
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IP protocol for PRRX2 antibody 23869-1-APDownload protocol
WB protocol for PRRX2 antibody 23869-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PRRX2 Polyclonal antibody (Cat# 23869-1-AP) has 5 citations published in Cancer Research, Redox Biology, Arteriosclerosis Thrombosis and Vascular Biology, Frontiers in Cell and Developmental Biology, and Cell Signaling. The research fields span prostate cancer (enzalutamide resistance), chondrocyte redox biology, aortic vulnerability in SMAD3 variant carriers, non-small cell lung cancer biomarker discovery, and hepatocellular carcinoma progression involving cuproptosis.

SpeciesApplicationTitle
humanWB

Cancer Res

A Genome-Wide CRISPR Activation Screen Identifies PRRX2 as a Regulator of Enzalutamide Resistance in Prostate Cancer

Authors - Yara Rodríguez
  • KO Validated
WB

Redox Biol

A reciprocal relationship between mitochondria and lipid peroxidation determines the chondrocyte intracellular redox environment

Authors - Madeline R Hines

Arterioscler Thromb Vasc Biol

Microfibril Denudation Marks Early Aortic Vulnerability in SMAD3 Variant Carriers: A Multigenerational Study.

Authors - Paulina M. Kowalewska
humanWB

Front Cell Dev Biol

QSOX2 Is an E2F1 Target Gene and a Novel Serum Biomarker for Monitoring Tumor Growth and Predicting Survival in Advanced NSCLC.

Authors - Yaqi Li
WB,ChIP

Cell Signal

Transcriptional regulation of ATOX1 by PRRX2 impacts the progression and cuproptosis of hepatocellular carcinoma

Authors - Ling Tang
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