Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human tonsillitis tissue, human colon cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25278-1-AP targets PR3/PRTN3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16765 Product name: Recombinant human PRTN3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 24-256 aa of BC096183 Sequence: RAAEIVGGHEAQPHSRPYMASLQMRGNPGSHFCGGTLIHPSFVLTAAHCLRDIPQRLVNVVLGAHNVRTQEPTQQHFSVAQVFLNNYDAENKLNDVLLIQLSSPANLSASVATVQLPQQDQPVPHGTQCLAMGWGRVGAHDPPAQVLQELNVTVVTFFCRPHNICTFVPRRKAGICFGDSGGPLICDGIIQGIDSFVIWGCATRLFPDFFTRVALYVDWIRSTLRRVEAKGRP Predict reactive species |
| Full Name | proteinase 3 |
| Calculated Molecular Weight | 256 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC096183 |
| Gene Symbol | PRTN3 |
| Gene ID (NCBI) | 5657 |
| RRID | AB_2880003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P24158 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PR3/PRTN3 antibody 25278-1-AP | Download protocol |
| WB protocol for PR3/PRTN3 antibody 25278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cathepsin C antibody (Cat# 25278-1-AP) has 6 total citations. These appear in Cell Mol Immunol, J Ovarian Res, iScience, Biomedicines, Front Cell Neurosci, and Toxicol In Vitro. Research fields span asthma immunology, ovarian cancer, acute lung injury, cardiovascular proteomics, blood-brain barrier disruption in meningitis, and VEGFR inhibitor-induced immune adverse events. Applications cited include WB, IF, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Cathepsin C orchestrates RSV-induced asthma exacerbation through the dual effect of monocyte-derived macrophages. | ||
J Ovarian Res Research on the process and molecular mechanism of inhibiting serous ovarian cancer by PRTN3 and its inhibitor Sivelestat | ||
iScience Cathepsin C-driven enhancement of neutrophil extracellular trap formation contributes to acute lung injury. | ||
Front Cell Neurosci Meningitic Escherichia coli-Induced Interleukin-17A Facilitates Blood-Brain Barrier Disruption via Inhibiting Proteinase 3/Protease-Activated Receptor 2 Axis. | ||
Toxicol In Vitro Activation of inflammasomes by tyrosine kinase inhibitors of vascular endothelial growth factor receptor: Implications for VEGFR TKIs-induced immune related adverse events. |
Reviews
What customers say
The single review for this antibody reports a 5-star rating. The user performed a Western Blot at a 1:1000 dilution on THP-1 cell lysate and confirmed the antibody worked well on their positive control. Overall, the feedback is positive, indicating reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Malgorzata (Verified Customer) (08-08-2022) | The antibody worked on my positive control - cell lysate from cultured THP-1 cells
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