PRDX3 Monoclonal antibody

• 

  WB analysis using 66810-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66810-1-Ig

  WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells.

• 

  WB analysis using 66810-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRDX3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66810-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66810-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66810-1-Ig (PRX3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66810-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (66810-1-Ig, Clone: 1F7F7 ) at dilution of 1:1700 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  Affinity and Kinetic Characterization of 66810-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66810-1-Ig against Human PRDX3 were performed. The affinity constant is 1.39 nM.

• Featured Product
• KD/KO Validated

PRDX3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66810-1-Ig

Clone No.1F7F7

Publications(3)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1F7F7, Antioxidant protein 1, AOP1, AOP-1, EC:1.11.1.24

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, LNCaP cells, HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:850-1:3400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

66810-1-Ig targets PRDX3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1062

  Product name: Recombinant human PRX3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-256 aa of BC007062

  Sequence: MAAAVGRLLRASVARHVSAIPWGISATAALRPAACGRTSLTNLLCSGSSQAKLFSTSSSCHAPAVTQHAPYFKGTAVVNGEFKDLSLDDFKGKYLVLFFYPLDFTFVCPTEIVAFSDKANEFHDVNCEVVAVSVDSHFSHLAWINTPRKNGGLGHMNIALLSDLTKQISRDYGVLLEGSGLALRGLFIIDPNGVIKHLSVNDLPVGRSVEETLRLVKAFQYVETHGEVCPANWTPDSPTIKPSPAASKEYFQKVNQ

• Full Name: peroxiredoxin 3
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC007062
• Gene Symbol: PRDX3
• Gene ID (NCBI): 10935
• RRID: AB_2882153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30048
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	WB	Free Radic Biol Med; Resveratrol and its derivative pterostilbene attenuate oxidative stress-induced intestinal injury by improving mitochondrial redox homeostasis and function via SIRT1 signaling.; Authors - Yanan Chen
rat	WB	Ecotoxicol Environ Saf; Mechanism of KAT2A regulation of H3K36ac in manganese-induced oxidative damage to mitochondria in the nervous system and intervention by curcumin; Authors - Yan Liu
mouse	WB	Int Immunopharmacol; Histone lactylation protects against sevoflurane-induced cognitive impairment by regulating YTHDF3/PRDX3 mediated microglial pyroptosis in neonatal mice; Authors - Yang Zhang