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PSAP Monoclonal antibody

PSAP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66473-1-Ig
Clone No.2F11B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator, A1 activator

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inhuman milk tissue, human milk, HepG2 cells, PC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66473-1-Ig targets PSAP in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1194

Product name: Recombinant human PSAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC001503

Sequence: MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEF

Predict reactive species
Full Name prosaposin
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_2881839
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP07602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 66473-1-IgDownload protocol
IHC protocol for PSAP antibody 66473-1-IgDownload protocol
IF protocol for PSAP antibody 66473-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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