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Presenilin-1-Specific Polyclonal antibody

Presenilin-1-Specific Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16163-1-AP

Synonyms

AD3, FAD, Presenilin-1, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182



Tested Applications

Positive WB detected inhuman kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16163-1-AP targets Presenilin-1-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name presenilin 1
Calculated molecular weight 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberNM_000021
Gene symbol PSEN1
Gene ID (NCBI) 5663
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.

Protocols

Product Specific Protocols
WB protocol for Presenilin-1-Specific antibody 16163-1-APDownload protocol
IHC protocol for Presenilin-1-Specific antibody 16163-1-APDownload protocol
IP protocol for Presenilin-1-Specific antibody 16163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neurosci Bull

PMID: 30632006

Authors - Yao Zhang
humanIHC

Oncotarget

Rat embryonic fibroblasts immortalized by MRPS18-2 protein are target for NK-cells.

Authors - Muhammad Mushtaq
human,ratWB

Neurotherapeutics

Direct Activation of Protein Phosphatase 2A (PP2A) by Tricyclic Sulfonamides Ameliorates Alzheimer's Disease Pathogenesis in Cell and Animal Models.

Authors - Hui Wei
mouse,humanWB

Cell Death Discov

BMP4 overexpression induces the upregulation of APP/Tau and memory deficits in Alzheimer's disease.

Authors - Xiaoqing Zhang
mouseWB

Neurosci Lett

Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.

Authors - Yasuteru Inoue
mouseWB

Cell Biol Int

Bone marrow mesenchymal stem cells-derived exosomes reduce Aβ deposition and improve cognitive function recovery in mice with Alzheimer's disease by activating sphingosine kinase/sphingosine-1-phosphate signaling pathway.

Authors - Xinhui Wang