PSG1 Polyclonal antibody

PSG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11809-1-AP

Synonyms

FL NCA 1/2, Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F

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Validation Data Gallery

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  WB analysis of human kidney using 11809-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11809-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 11809-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 11809-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 11809-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSG1 antibody (11809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: human kidney tissue, MCF-7 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

11809-1-AP targets PSG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSG1 fusion protein Ag2468
• Full Name: pregnancy specific beta-1-glycoprotein 1
• Calculated Molecular Weight: 426 aa, 48 kDa
• Observed Molecular Weight: 72 kDa, 36 kDa
• GenBank Accession Number: BC022338
• Gene Symbol: PSG1
• Gene ID (NCBI): 5669
• RRID: AB_2171045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Fertil Steril; Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation.; Authors - Jay M Bolnick