PSG3 Polyclonal antibody

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  WB analysis using 15375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15375-1-AP (PSG3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 15375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15375-1-AP (PSG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15375-1-AP (PSG3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSG3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15375-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Carcinoembryonic antigen SG5, Pregnancy-specific beta-1-glycoprotein 3, Pregnancy-specific glycoprotein 3, PS beta G 3, PS-beta-G-3

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15375-1-AP targets PSG3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1148

  Product name: Recombinant human PSG3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 129-428 aa of BC005924

  Sequence: DGTRGETGHFTFTLYLETPKPSISSSNLYPREDMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLQLSKNKRTLFLFGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLAFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIQDRYGGIRSYPVTLNVLYGPDLPRIYPSFTYYHSGENLYLSCFADSNPPAEYSWTINGKFQLSGQKLFIPQITTKHSGLYACSVRNSATGMESSKSMTVEVSAPSGTGHLPGLNPL

• Full Name: pregnancy specific beta-1-glycoprotein 3
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa, 64 kDa
• GenBank Accession Number: BC005924
• Gene Symbol: PSG3
• Gene ID (NCBI): 5671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16557
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human pregnancy-specific glycoproteins (PSGs) are a group of molecules that are mainly produced by the placental syncytiotrophoblasts during pregnancy (PMID: 1644821). The calculated molecular weight of PSG3 is 48 kDa. The 64 kDa band is its glycosylated protein form (PMID: 7851896).