PSKH2 Polyclonal antibody

PSKH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18813-1-AP

Synonyms

Serine/threonine-protein kinase H2, PSK-H2, PSK H2, Protein serine kinase H2, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis of HepG2 using 18813-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18813-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18813-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 18813-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 18813-1-AP

  IP result of anti-PSKH2 (IP:18813-1-AP, 4ug; Detection:18813-1-AP 1:500) with HepG2 cells lysate 3400ug.

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  IHC staining of human kidney using 18813-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18813-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18813-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18813-1-AP (PSKH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, human heart tissue, human testis tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18813-1-AP targets PSKH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein serine kinase H2
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC126182
• Gene Symbol: PSKH2
• Gene ID (NCBI): 85481
• RRID: AB_2171244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSKH2 belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. PSKH2 catalytic the reaction: ATP + a protein = ADP + a phosphoprotein.