PSMA2 Polyclonal antibody

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PSMA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14377-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HC3, Proteasome subunit alpha type-2, PSC3

• 

  WB analysis using 14377-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14377-1-AP

  WB result of PSMA2 antibody (14377-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA2 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 14377-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14377-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 14377-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 14377-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14377-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 14377-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 14377-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14377-1-AP (PSMA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 14377-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human skeletal muscle using 14377-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 14377-1-AP (PSMA2 antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14377-1-AP

  Immunofluorescent analysis of HepG2 cells using 14377-1-AP (PSMA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, mouse kidney tissue, PC-3 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14377-1-AP targets PSMA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5753

  Product name: Recombinant human PSMA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-234 aa of BC047697

  Sequence: MAERGYSFSLTTFSPSGKLVQIEYALAAVAGGAPSVGIKAANGVVLATEKKQKSILYDERSVHKVEPITKHIGLVYSGMGPDYRVLVHRARKLAQQYYLVYQEPIPTAQLVQRVASVMQEYTQSGGVRPFGVSLLICGWNEGRPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNEDLELEDAIHTAILTLKESFEGQMTEDNIEVGICNEAGFRRLTPTEVKDYLAAIA

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC047697
• Gene Symbol: PSMA2
• Gene ID (NCBI): 5683
• RRID: AB_2878050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25787
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

Publications

What published studies show

The PSMA2 Polyclonal antibody (Cat# 14377-1-AP) has 4 total citations published in Acta Biomaterialia, EMBO Reports, Journal of Gastroenterology, and Journal of Ovarian Research. The associated research fields include tumor targeted nanotherapy, bile and liver metabolism, chronic pancreatitis with pancreatic fibrosis, and ovarian cancer prognosis based on tumor stem cell-related gene signatures.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Acta Biomater; Pushpin-like nanozyme for plasmon-enhanced tumor targeted therapy; Authors - Baofu Ma
	WB	EMBO Rep; MAPL loss dysregulates bile and liver metabolism in mice; Authors - Vanessa Goyon
human	WB	J Gastroenterol; Corynoline protects chronic pancreatitis via binding to PSMA2 and alleviating pancreatic fibrosis; Authors - Pengyuan Wang
human	IHC	J Ovarian Res; Development and clinical validation of a seven-gene signature based on tumor stem cell-related genes to predict ovarian cancer prognosis; Authors - Guangwei Wang