PSMA3 Polyclonal antibody

PSMA3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11887-1-AP

Synonyms

20S proteasome, HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3

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Validation Data Gallery

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  WB analysis of A549 using 11887-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11887-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 11887-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, PC-12 cells, human lung tissue, COS-7 cells, RAW 264.7 cells, mouse spleen tissue, rat brain tissue, HeLa cells, NIH/3T3 cells, mouse liver tissue
• Positive IHC detected in: human lymphoma tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Product Information

11887-1-AP targets PSMA3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMA3 fusion protein Ag2495
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 3
• Calculated Molecular Weight: 255 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC029402
• Gene Symbol: PSMA3
• Gene ID (NCBI): 5684
• RRID: AB_2171420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	ELISA	Free Radic Biol Med; Identification of an unstable 4-hydroxynoneal modification on the 20S proteasome subunit α7 by recombinant antibody technology.; Authors - Jesper Just
mouse	IF	Clin Transl Med; FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination.; Authors - Yang Wang
		Cell Mol Life Sci; The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S.; Authors - Diego Sbardella
human	WB	ChemMedChem; Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity.; Authors - Anna Maria Santoro
human	WB	Research (Wash D C); SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage; Authors - Fengli Wang
human	WB	iScience; Guanylate-binding protein 1 modulates proteasomal machinery in ovarian cancer; Authors - Dhanir Tailor