PSMA4 Monoclonal antibody, PBS Only

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• PSMA4 Monoclonal antibody, PBS Only (68203-1-PBS)

PSMA4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68203-1-PBS

Clone No.2E6E4

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2E6E4, HC9, Proteasome subunit alpha type-4, PSC9

• 

  WB analysis using 68203-1-Ig (same clone as 68203-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68203-1-Ig (PSMA4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 68203-1-Ig (same clone as 68203-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68203-1-Ig (PSMA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 68203-1-Ig (same clone as 68203-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMA4 antibody (68203-1-Ig, Clone: 2E6E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

68203-1-PBS targets PSMA4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32626

  Product name: Recombinant human PSMA4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-237 aa of BC056249

  Sequence: MEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 4
• Calculated Molecular Weight: 237 aa, 27 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC056249
• Gene Symbol: PSMA4
• Gene ID (NCBI): 5685
• RRID: AB_2935291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P25789
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSMA4 (Proteasome Subunit Alpha 4) is a gene that encodes the alpha-4 subunit of the 20S proteasome core particle, which forms part of the outer alpha-ring structure of the barrel-shaped 20S complex. As an integral component of the 26S proteasome, PSMA4 participates in ATP- and ubiquitin-dependent protein degradation, a process essential for the regulation of cell cycle progression, signal transduction, and protein quality control. In addition, the 20S core particle can associate with regulatory complexes such as PA28 or PA200 to mediate ubiquitin-independent protein degradation, which plays important roles in pathways including antigen peptide generation and spermatogenesis. Dysregulation of PSMA4 expression or function has been implicated in various pathological conditions, including cancer and neurodegenerative diseases, highlighting its critical role in maintaining cellular proteostasis.