PSMA5 Polyclonal antibody

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  WB analysis using 31384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31384-1-AP (PSMA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 31384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 31384-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31384-1-AP (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 31384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (31384-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

PSMA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31384-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Proteasome subunit alpha type-5, PSC5, ZETA

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse liver tissue, mouse thymus tissue
• Positive IHC detected in: mouse brain tissue, rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

31384-1-AP targets PSMA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34928

  Product name: Recombinant human PSMA5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-241 aa of BC103751

  Sequence: MFLTRSEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGIQTSEGVCLAVEKRITSPLMEPSSIEKIVEIDAHIGCAMSGLIADAKTLIDKARVETQNHWFTYNETMTVESVTQAVSNLALQFGEEDADPGAMSRPFGVALLFGGVDEKGPQLFHMDPSGTFVQCDARAIGSASEGAQSSLQEVYHKSMTLKEAIKSSLIILKQVMEEKLNATNIELATVQPGQNFHMFTKEELEEVIKDI

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 5
• Calculated Molecular Weight: 241 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC103751
• Gene Symbol: PSMA5
• Gene ID (NCBI): 5686
• RRID: AB_3669959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P28066
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Discov Oncol; Unveiling the role of PSMA5 in glioma progression and prognosis; Authors - Wei Liu ; KD Validated
human	WB	PLoS Genet; Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1; Authors - Chenliang Zhang