PSMA7 Monoclonal antibody, PBS Only

• 

  WB analysis using 67817-1-Ig (same clone as 67817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

  WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

  WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells. This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67817-1-Ig (same clone as 67817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Caco-2 using 67817-1-Ig (same clone as 67817-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67817-1-Ig (same clone as 67817-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67817-1-Ig (same clone as 67817-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug PSMA7 Monoclonal antibody (67817-1-Ig, Clone:1F2C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67817-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PSMA7 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 67817-1-PBS

Clone No.1F2C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

C6, HSPC, Proteasome subunit alpha type-7, Proteasome subunit RC6-1, RC6 1

---

Product Information

67817-1-PBS targets PSMA7 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7821

  Product name: Recombinant human PSMA7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-248 aa of BC004427

  Sequence: MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS

• Full Name: proteasome (prosome, macropain) subunit, alpha type, 7
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC004427
• Gene Symbol: PSMA7
• Gene ID (NCBI): 5688
• RRID: AB_2918580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O14818
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).