PSMB10 Monoclonal antibody

PSMB10 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8C1

Cat no : 66403-1-Ig

Synonyms

beta2i, LMP10, Low molecular mass protein 10, Macropain subunit MECl 1, MECL1, Proteasome MECl 1, Proteasome subunit beta 2i, PSMB10



Tested Applications

Positive WB detected inRaji cells, Ramos cells, U-937 cells, rat thymus tissue, human spleen tissue, pig thymus tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66403-1-Ig targets PSMB10 in WB, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PSMB10 fusion protein Ag8835
Full Name proteasome (prosome, macropain) subunit, beta type, 10
Calculated Molecular Weight 273 aa, 29 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017198
Gene Symbol PSMB10
Gene ID (NCBI) 5699
RRIDAB_2881777
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMB10(Proteasome subunit beta type-10) is also named as LMP10, MECL1.It belongs to the peptidase T1B family and is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.

Protocols

Product Specific Protocols
WB protocol for PSMB10 antibody 66403-1-IgDownload protocol
IHC protocol for PSMB10 antibody 66403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols