Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55143-1-AP targets PSMB11 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 11 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | NM_001099780 |
| Gene Symbol | PSMB11 |
| Gene ID (NCBI) | 122706 |
| RRID | AB_10863288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A5LHX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PSMB11 antibody 55143-1-AP | Download protocol |
| IHC protocol for PSMB11 antibody 55143-1-AP | Download protocol |
| WB protocol for PSMB11 antibody 55143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PSMB11 Polyclonal antibody (Cat# 55143-1-AP) has 1 total citation. The citation appears in Oncology Letters, a peer-reviewed journal. The associated research field is oncology/hematology, specifically addressing metastatic thymoma complicated by pure red cell aplasia, focusing on chemotherapy outcomes and transfusion dependence in a case report.
| Species | Application | Title |
|---|---|---|
Oncol Lett Initial chemotherapy decreases transfusion dependence and enables definitive therapy in metastatic thymoma complicated by pure red cell aplasia: A case report. |
Reviews
What customers say
One review was submitted for this antibody in a Flow Cytometry application at a 1:500 dilution. The reviewer tested it on thymus cells and Psmb11 knockout mice but reported being unable to obtain specific staining. The signal observed in the knockout mice was comparable to that seen in wildtype thymocytes or thymus epithelial cells, suggesting the antibody did not differentiate between genotypes as expected.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anja (Verified Customer) (03-16-2022) | We tested the antibody in different dilutions on different cells of the thymus and Psmb11 knockout (KO) mice and could not obtain a specific staining with the antibody. In our hands the signal in the Psmb11-KO mice was comparable to the one obtained from either thymocytes or thymus epithelial cells of Wildtype mice .
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