PSMB3 Polyclonal antibody

PSMB3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15983-1-AP

Synonyms

Proteasome subunit beta type-3, Proteasome component C10-II, HC10 II

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Validation Data Gallery

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  WB analysis using 15983-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15983-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15983-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15983-1-AP (PSMB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15983-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15983-1-AP (PSMB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15983-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15983-1-AP (PSMB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 15983-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PSMB3 antibody (15983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293T cells, mouse testis tissue, rat testis tissue, HeLa cells, MCF-7 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15983-1-AP targets PSMB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB3 fusion protein Ag8784
• Full Name: proteasome (prosome, macropain) subunit, beta type, 3
• Calculated Molecular Weight: 205 aa, 23 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC013008
• Gene Symbol: PSMB3
• Gene ID (NCBI): 5691
• RRID: AB_2172027
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.