PSMB6 Polyclonal antibody

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• PSMB6 Polyclonal antibody (11684-2-AP)

PSMB6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11684-2-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.4.25.1, LMPY, Proteasome subunit beta type-6, Y

• 

  WB analysis using 11684-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 11684-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11684-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 11684-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of fetal human brain using 11684-2-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Caco-2 using 11684-2-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human gliomas using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11684-2-AP (PSMB6 antibody) at dilution of 1:50 (under 25x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 11684-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB6 antibody (11684-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Caco-2 cells, fetal human brain tissue, human liver tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11684-2-AP targets PSMB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2296

  Product name: Recombinant human PSMB6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-239 aa of BC000835

  Sequence: MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA

• Full Name: proteasome (prosome, macropain) subunit, beta type, 6
• Calculated Molecular Weight: 239 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000835
• Gene Symbol: PSMB6
• Gene ID (NCBI): 5694
• RRID: AB_2172199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28072
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).

Publications

What published studies show

The PSMB6 Polyclonal antibody (Cat# 11684-2-AP) has 7 citations published in journals including Nature Communications, JHEP Reports, Biochemical Pharmacology, Journal of Biological Chemistry, PLoS Genetics, Frontiers in Genetics, and Cell Physiology and Biochemistry. Research fields span lung adenocarcinoma metastasis, APAP-induced liver injury, atrial fibrillation, hepatocellular carcinoma, ubiquitin-independent proteasomal degradation, immune regulation and prognostic modeling, and acute cold stress response.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Nat Commun; Multi-omics with dynamic network biomarker algorithm prefigures organ-specific metastasis of lung adenocarcinoma; Authors - Xiaoshen Zhang
mouse	WB	JHEP Rep; Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice; Authors - Chunhong Liu
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie
mouse	WB	J Biol Chem; Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes; Authors - Xinzhi Li
human	WB,IF	PLoS Genet; Nuclear p62/SQSTM1 facilitates ubiquitin-independent proteasomal degradation of BMAL1; Authors - Chenliang Zhang
human	WB	Front Genet; Immune regulation and prognostic prediction model establishment and validation of PSMB6 in lung adenocarcinoma; Authors - Haiyang Zhao