PSMB6 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86491-1-RR (same clone as 86491-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86491-1-RR (PSMB6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86491-1-RR (same clone as 86491-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMB6 antibody (86491-1-RR, Clone: 251232G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86491-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86491-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86491-1-RR against Human PSMB6 were performed. The affinity constant is below 1 pM.

PSMB6 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86491-1-PBS

Clone No.251232G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:3.4.25.1, LMPY, Proteasome subunit beta type-6, Y

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Product Information

86491-1-PBS targets PSMB6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2296

  Product name: Recombinant human PSMB6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-239 aa of BC000835

  Sequence: MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDKLTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA

• Full Name: proteasome (prosome, macropain) subunit, beta type, 6
• Calculated Molecular Weight: 239 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000835
• Gene Symbol: PSMB6
• Gene ID (NCBI): 5694
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28072
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).