PSMB7 Polyclonal antibody

PSMB7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14727-1-AP

Synonyms

20S proteasome, Macropain chain Z, Proteasome subunit beta type 7, Proteasome subunit Z, PSMB7

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Validation Data Gallery

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  WB analysis of Raji using 14727-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14727-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14727-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14727-1-AP (PSMB7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 14727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 14727-1-AP (PSMB7 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Raji cells, A549 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

14727-1-AP targets PSMB7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB7 fusion protein Ag6486
• Full Name: proteasome (prosome, macropain) subunit, beta type, 7
• Calculated Molecular Weight: 29.9 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC000509
• Gene Symbol: PSMB7
• Gene ID (NCBI): 5695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Androgen receptor polyglutamine expansion drives age-dependent quality control defects and muscle dysfunction.; Authors - Samir R Nath
rat	WB	Int J Mol Sci; Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.; Authors - Wenjuan Zhang
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie