PSMC5 Polyclonal antibody

PSMC5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14752-1-AP

Synonyms

p45, p45/SUG, Proteasome subunit p45, PSMC5, Rpt6, S8, SUG1, TBP10, TRIP1

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Validation Data Gallery

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  WB analysis using 14752-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14752-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14752-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14752-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14752-1-AP (PSMC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14752-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14752-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14752-1-AP (PSMC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14752-1-AP targets PSMC5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMC5 fusion protein Ag6497
• Full Name: proteasome (prosome, macropain) 26S subunit, ATPase, 5
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 45-49 kDa
• GenBank Accession Number: BC002367
• Gene Symbol: PSMC5
• Gene ID (NCBI): 5705
• RRID: AB_2878077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.